Five assassins aboard a fast moving bullet train find out their missions have something in common.

Trained killer Ladybug wants to give up the life but is pulled back in by his handler Maria Beetle in order to collect a briefcase on a bullet train heading from Tokyo to Morioka. On board are fellow assasins Kimura, the Prince, Tangerine, and Lemon. Once on board the five assasins discover that their objectives are all connected.

Foul-mouthed mutant mercenary Wade Wilson (a.k.a. Deadpool), brings together a team of fellow mutant rogues to protect a young boy with supernatural abilities from the brutal, time-traveling cyborg Cable.